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KMID : 0438619930170010001
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Journal of Oral Biology
1993 Volume.17 No. 1 p.1 ~ p.6
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Calcium Modulation of Growth and Differentiation of Immortal Human Oral Keratinc cytes
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Min Byung-Moo
Kim Kyung-Ae
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Abstract
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Modification of the medium calcium concentration markedly modulates the pattern of proliferation and differentiation of cultured epidermal cells in human and murine. Correlation of thr difference in the molecular weights of some of the keratins with those of NBT reduction abilitics of human oral keratinocytes was studied in relation to its differentiation. Immortal human oral keratinocytes were grown in a serum-free keratinocyte growth medium containing 0.15, 0.3, 0.6 or 1.2 mM calcium for 3 days and assayed for the DNA synthesis, NBT reduction, and keratin profiles. The addition of calcium in culture medium produced altered morphetogy, enhanced percrntage of NBT-positive cells and inhibited de novc DHA synthesis, showing that extracellular catcium induced the differentiation of immortal oral keratiuocytes. In low-calcium-grown cells the low molecular weight keratins were observed, however, the addition of calcium to low-calcium-grown cells resulted in induction of the high molecular weight keratins. These results indicate that the increase of the molecular weights of some of the keratins correlates with the degree of the differentiation of oral kerarinocytes. We guggest a possibility that the change of keratin profiles mnay be used as a molecular marker of calcium-induced keratinocyte differentiation.
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